Reduced Vs Non Reduced Sds Page

Ferritin, Light Chain (FTL) Monoclonal Antibody (FTL, 2338R

Reduced Vs Non Reduced Sds Page. A reducing agent can break disulfide bonds, and for a majority of proteins, this will not. If we had a heterotrimer, we would only see one band.

Ferritin, Light Chain (FTL) Monoclonal Antibody (FTL, 2338R
Ferritin, Light Chain (FTL) Monoclonal Antibody (FTL, 2338R

So in reducing sds, you add bme or another. A reducing agent can break disulfide bonds, and for a majority of proteins, this will not. Samples can be reduced or nonreduced. If we had a heterotrimer, we would only see one band.

Samples can be reduced or nonreduced. If we had a heterotrimer, we would only see one band. So in reducing sds, you add bme or another. A reducing agent can break disulfide bonds, and for a majority of proteins, this will not. Samples can be reduced or nonreduced.