Urea Page Gel. Nucleic acids from 50 to 2,000 bp to are efficiently separated on tbe gels. Add 25 μl temed and 50 μl 25% aps.
TeamEPFL/Results/Toehold
Add 25 μl temed and 50 μl 25% aps. Pour gel to ~ 0.5 cm from top. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. Web tbe gels are used for dsdna analysis, to assess the purity of pcr products and for rnase protection assays. Insert clean, dry comb at an angle to prevent trapping of bubbles. Nucleic acids from 50 to 2,000 bp to are efficiently separated on tbe gels. Push all the way down, but don't trap any bubbles. For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following:
For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: Push all the way down, but don't trap any bubbles. Pour gel to ~ 0.5 cm from top. Web tbe gels are used for dsdna analysis, to assess the purity of pcr products and for rnase protection assays. Add 25 μl temed and 50 μl 25% aps. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. Nucleic acids from 50 to 2,000 bp to are efficiently separated on tbe gels. For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: Insert clean, dry comb at an angle to prevent trapping of bubbles.
